By Plamen Demirev, Todd R. Sandrin

ISBN-10: 3319260685

ISBN-13: 9783319260686

ISBN-10: 3319260707

ISBN-13: 9783319260709

In the final region century, advances in mass spectrometry (MS) were on the vanguard of efforts to map complicated organic structures together with the human metabolome, proteome, and microbiome. All of those advancements have allowed MS to turn into a well-established molecular point know-how for microorganism characterization. MS has verified its massive virtue as a speedy, actual, and cost-efficient process for microorganism id, in comparison to traditional phenotypic strategies. within the final numerous years, functions of MS for microorganism characterization in learn, medical microbiology, counter-bioterrorism, nutrients safeguard, and environmental tracking were documented in millions of guides. Regulatory our bodies in Europe, the united states, and somewhere else have licensed MS-based assays for infectious ailment diagnostics. As of mid-2015, greater than 3300 advertisement MS platforms for microorganism identity were deployed world wide in hospitals and scientific labs.

While prior paintings has coated broader ways in utilizing MS to symbolize microorganisms on the species point or above, this ebook makes a speciality of strain-level and subtyping purposes. In twelve person chapters, innovators, leaders and practitioners within the box from worldwide have contributed to a accomplished evaluation of present and next-generation methods for MS-based microbial characterization on the subspecies and pressure degrees. Chapters comprise up to date reference lists in addition to web-links to databases, prompt software program, and different precious tools.

The emergence of recent, antibiotic-resistant lines of human or animal pathogens is of impressive main issue not just to the medical and scientific groups, yet to most people to boot. advancements of novel MS-based assays for speedy identity of traces of antibiotic-resistant microorganisms are reviewed within the e-book to boot. Microbiologists, bioanalytical scientists, infectious ailment experts, medical laboratory and public overall healthiness practitioners in addition to researchers in universities, hospitals, executive labs, and the pharmaceutical and biotechnology industries will locate this publication to be a well timed and priceless source.

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Extra resources for Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance

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Chang C-J, Lin J-H, Chang K-C, Lai M-J, Rohini R, Hu A. Diagnosis of Β-Lactam resistance in Acinetobacter baumannii using shotgun proteomics and LC-Nano-Electrospray ionization ion trap mass spectrometry. Anal Chem. 2013;85:2802–8. Chennamaneni NK, Kumar AB, Barcenas M, Spáčil Z, Scott CR, Tureček F, Gelb MH. Improved reagents for newborn screening of mucopolysaccharidosis Types I, II, and VI by tandem mass spectrometry. Anal Chem. 2014;86(9):4508–14. Clark AE, Kaleta EJ, Arora A, Wolk DM. Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology.

2013; Li et al. 2014; Deng et al. 2014). It is natural that the use of MALDI-MS has been extended to the identification of bacteria at the strain level and for the differentiation of antibiotic-resistant strains. , sample preparation). The reader is also referred to more comprehensive literature reviews on the detection of microorganisms at the strain level and/or antibiotic-resistant strains (Croxatto et al. 2012; Sandrin et al. 2013; Hrabak et al. 2013). Differentiation of bacteria beyond the species level with MS is challenging since the detected number of unique or characteristic biomarkers decreases as the similarity between microorganisms increases.

Where carbapenemase activity was detected using MALDI-MS in viable intact cells of Enterobacteriaceae and Pseudomonas spp. (Hrabak et al. 2011) and later expanded to Acinetobacter baumannii (Hrabák et al. 2012). , meropenem). This suspension is incubated for ~2 h at 35 °C, in which period meropenem molecules are enzymati- 40 F. Basile and R. K. Mignon cally hydrolyzed at the β-lactam moiety followed by decarboxylation. The bacteria suspension is then centrifuged and the supernatant is analyzed by MALDI-MS.

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Applications of Mass Spectrometry in Microbiology: From Strain Characterization to Rapid Screening for Antibiotic Resistance by Plamen Demirev, Todd R. Sandrin

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