By S. Morse
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Spectral conditions: 85 mW of 785 nm, 1 min acquisition time, 8 cm-1 resolution. Acetic acid produced several peaks, notably at 925, 1340, and 1405 cm-1. Subtraction of the acetic acid spectrum reveals a standard SER spectrum of DPA (Fig. 12C). The SER spectral intensity of DPA in acetic acid as a function of concentration is very similar to that previously measured in water. 14 in water, Table 2). Fig. 13 shows SER spectra of four orders of magnitude of DPA Fig. 13. SERS of DPA in acetic acid at concentrations of A) 1, B) 10, C) 100, and D) 1000 pg/µL.
Spectral conditions: 150 mW of 785 nm, 8 cm-1 resolution. Although, these initial measurements clearly demonstrated the potential of rapid spore analysis using SERS, the approach had two significant limitations. First, the sensitivity is insufficient, and second the use of a hot reagent severely limits its practical use in the field. g. ), separately and as mixtures that could digest spores rapidly at room temperature, and make available all or nearly all of the DPA for SERS detection. Eventually, it was found that glacial acetic acid had the appropriate properties.
Aureus (Table 1). Using the Clustal W program, the amino acid sequences of the SEs were aligned and evolutionary distances determined. , 2003). The first two groups contain the classical toxins SEA, SED, SEE (Group 1) and SEB, SEC (Group 2) in addition to newly identified SEs; Group 3 contains only newly identified toxins. There is some similarity in structure in that Groups 1, 2, and 5 have a disulfide bond while Group 3 and Group 4 (TSST-1) do not. , 2010). Some bind zinc in the concave β sheet of the C terminal domain while others bind zinc in a cleft between the two domains.
Bioterrorism by S. Morse
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